24589-78-4 | N-Methyl-N-(trimethylsilyl)trifluoroacetamide

CAS:24589-78-4 | N-Methyl-N-(trimethylsilyl)trifluoroacetamide ,Description

N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) is a versatile, non-volatile, and non-toxic reagent used in various laboratory experiments and applications. It is a colorless liquid with a boiling point of approximately 150°C and a melting point of approximately -50°C. MSTFA is used in a variety of scientific research applications, including organic synthesis, analytical chemistry, and biochemistry. 
 

Scientific Research Applications

Analytical Methods of Steroid Estrogens

MSTFA is widely used in the environmental science and ecological toxicology field, particularly for derivatizing hydroxyl and ketone groups in steroids. It aids in the formation of trimethylsilyl (TMS) derivatives, which are crucial for analyzing steroid estrogens in environmental samples like water and sediment (Bi, 2015).

Derivatization for Gas Chromatography

MSTFA is employed for the trifluoroacetylation of amine, hydroxyl, and thiol groups. This process creates neutral trifluoroacetamides, which are volatile and elute early in gas chromatography, enhancing analytical accuracy (Donike, 1973).

Mass Spectrometric Estimation

In combined gas chromatography and mass spectrometry, MSTFA is used for preparing derivatives of phenolalkylamines, offering stability and aiding in detailed analysis (Donike, 1975).

Derivatization of Iminodicarboxylic Acids

MSTFA is part of a derivatizing reagent mix used for the N-trifluoroacetylation and O-trimethylsilylation of iminodicarboxylic acids, leading to derivatives that can be analyzed via gas chromatography-mass spectrometry (Kawashiro et al., 1985) .

Suitability as a Derivatization Reagent

A study evaluated the suitability of MSTFA for derivatizing hormones like estrone and 17alpha-ethinylestradiol, indicating its potential limitations in certain contexts (Shareef et al., 2004).

Stereospecific Derivatization

MSTFA is used for protecting hydroxyl groups in the analysis of amphetamines, phenol alkylamines, and hydroxyamines. It facilitates the separation of enantiomers in capillary gas-liquid chromatography (Shin & Donike, 1996).

Hydrophobicity Improvement in Ti-MCM-41

MSTFA has been employed for the trimethylsilylation of Ti-MCM-41, enhancing its hydrophobicity, which is beneficial in certain chemical processes (Bu & Rhee, 2000).

Comprehensive GC-MS Profiling

MSTFA's role in gas chromatography-mass spectrometry-based metabolome preparation protocols was studied, aiming for global, reliable, and reproducible metabolite profiling of leukemia cells (Yue et al., 2018).

Zinc-Catalyzed Dehydration of Amides

MSTFA was used as a dehydration reagent in the zinc-catalyzed conversion of amides to nitriles, showcasing its utility in organic synthesis (Enthaler & Inoue, 2012).

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