63812-15-7 | 2-Fluoroisobutyric acid

CAS:63812-15-7 | 2-Fluoroisobutyric acid ,Description

2-Fluoroisobutyric acid (2-FIB) is an organic compound that is used in a variety of scientific research applications. It is a derivative of isobutyric acid, with a fluorine atom replacing the hydrogen atom on the second carbon. 2-FIB has a number of biochemical and physiological effects, and has been used in a wide range of laboratory experiments. In 
 

Scientific Research Applications

Fluorescent Amino Acids in Chemical Biology

Fluorescent amino acids, including derivatives of 2-fluoroisobutyric acid, are crucial in chemical biology. They are used to construct fluorescent macromolecules like peptides and proteins, enabling the study of biological systems non-invasively. These amino acids help in tracking protein-protein interactions and imaging nanoscopic events in real-time with high spatial resolution (Cheng, Kuru, Sachdeva, & Vendrell, 2020).

Optical Oxygen Probing in Neuroscience

In neuroscience, derivatives of 2-fluoroisobutyric acid, like pyrenebutyric acid, have been used as probes for monitoring intracellular oxygen tension in cerebral cortex studies. This method provides a non-toxic, non-invasive, and non-consumptive way of monitoring, overcoming limitations of traditional techniques (Mitnick & Jöbsis, 1976).

PET Tracers in Cancer Imaging

2-Fluoroisobutyric acid derivatives, like 2-[18F]-fluorobutyric acid, are explored as PET tracers for imaging prostate cancer. These compounds show promise in detecting tumors, with good accumulation in the tumor and clearance from non-target organs (Pillarsetty, Punzalan, Ku, & Larson, 2009).

Biophysical Studies with Fluorinated Amino Acids

Fluorinated amino acids, including 2-fluoroisobutyric acid derivatives, are valuable in biophysical studies. They provide insights into protein structure and dynamics through 19F NMR chemical shifts. These amino acids are used to study the role of proton transfer and tautomeric states in enzymatic mechanisms (Kasireddy, Ellis, Bann, & Mitchell-Koch, 2017).

Genetically Encoded Fluorescent Amino Acids

2-Fluoroisobutyric acid derivatives, like dansylalanine, have been genetically encoded in organisms like Saccharomyces cerevisiae. These fluorophores are introduced into proteins at defined sites, facilitating the study of protein unfolding and other dynamic processes (Summerer, Chen, Wu, Deiters, Chin, & Schultz, 2006).

Biosynthesis from Renewable Carbon

Research on the biosynthesis of compounds like 2-hydroxyisobutyric acid, closely related to 2-fluoroisobutyric acid, from renewable carbon sources, highlights the potential of biotechnology in producing valuable chemical building blocks. This approach is significant for developing sustainable chemical processes (Rohwerder & Müller, 2010).

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